Discipline: Biological Sciences
Subcategory: Cell and Molecular Biology
Aminat Animashaun - Alabama A&M University
Co-Author(s): Clare Johnson and Heather Bradshaw, PhD., Indiana University
This study investigates the lipodomic profiles in BV2 microgilal cells and determines the effects of GPR55 antagonists. More specifically, looking at how lipid molecules change as a result of this GPR55 antagonism. Microgilal cells are primary immune cells that are located in the central nervous system. The GPR55 is a G-Protein Couple Receptor that is activated by THC and endogenous cananabinoinds. GPR55 in GPR55 KO mice were studied across multiple brain regions in which prostaglandins were reduced. The antagonist of the GPR55 receptor that is used is to investigate the lipodomic profiles in BV2 microgilal cells is ML193. To test this antagonistic effect and investigate the lipodomic profiles, cells will be treated with 10?M of ML 193 or vehicle, lipid extraction/partial purification performed, mass spectrometric analysis performed, MS data analyzed, pellets weighed, and final stats run. Treatment of GPR55 antagonist ML 193 results yielded a cellular effect on the BV2 microglial lipidome. The lipids that demonstrated significant or trending effect were N-docosahexaenoyl glycine and N-docosahexaenoyl enthanolamine. N-docosahexaenoyl glycine and N-docosahexaenoyl enthanolamine had significant decreases. The main effect of the GPR55 KO in mice was a significant decrease in ProstaglandinE2, having a significant decrease in 5 of the 6 brain regions studied. This effect was not observed with ML193 in the BV2 cells suggesting that either that microglial cells play a limited role in the levels of PGE2 in the brain or that long-term inhibition is required to drive these changes. References: D. McHugh, J. Page, E. Dunn, and H. B. Bradshaw, D9 -Tetrahydrocannabinol and N-arachidonyl glycine are full agonists at GPR18 receptors and induce migration in human endometrial HEC-1B cells British Journal of Pharmacology, 165: 2414-2424. doi: 10.111/j.1476-5381.2011.01497. Dr. Green, Kim. (2019).Microglial function in the Healthy Brain. Retrieved from https://faculty.sites.uci.edu/kimgreen/bio/microglia-in-the-healthy-brain H. B. Bradshaw, N. Rimmerman, J. F. Krey, and J. M. Walker, Sex and hormonal cycle differences in rat brain levels of pain-related cannabimimetic lipid mediators, American Journal of Physiology Regulatory Integrative and Comparative Physiology, vol. 291, no. 2, pp. R349?R358, 2006. J. M. Stuart, J. J. Paris, C. Frye, and H. B. Bradshaw, Brain Levels of Prostaglandins, Endocannabinoids, and Related Lipids Are Affected by Mating Strategies, International Journal of Endocrinology, vol. 2013, Article ID 436252, 14 pages, 2013.Lu, Hui-Chen & Mackie, Ken. Funder Acknowledgement(s): I thank Dr. Heather Bradshaw for help in the field and serving as the principal investigator. Dr. Jack L. Schmit at Indiana University provided instrumental and logistic support. Funding was provided by Indiana University through the STEM Scholars Institute Program. Faculty Advisor/ Mentor: Dr. Marius Schamschula
Funder Acknowledgement(s): Dr. Heather Bradshaw (Principal Investigator); Clare Johnson (Graduate Student); Dr. Jack Schmit (Program Coordinator)
Faculty Advisor: Dr. Marius Schamschula, email@example.com
Role: In this research project, I mainly worked under a graduate student extracting data and conducting data analysis.