Discipline: Biological Sciences
Room: Exhibit Hall A
Rajon Scott - Howard University
CD44 and LRG1 are both genes that are related to malignant tumors. Increased expression of these genes lead to greater risk of malignancy, and the promotion of metastasis of cancerous tumors. This study sought to standardize the protocol to identify the CD44 and LRG1 genes. Identification was done by PCR amplification and gel electrophoresis using samples of SH-SY5Y cells. Due to insufficient time to conduct research no significant conclusions were made, however LRG1 was identified using PCR. If more time as alloted quantification by real-time PCR would have been performed to quantify the expression of the LRG1 gene. Future directions of the study would include taking samples from patients with different types of cancer with metastasis and without metastasis to identify the presence and expression of the CD44 and LRG1 genes.
Funder Acknowledgement(s): NSF HBCU-UP
Faculty Advisor: Dra. Elizabeth, email@example.com
Role: I completed the bench work for the PCR's and gel electrophoresis