Discipline: Biological Sciences
Makayla Turner - Claflin University
Co-Author(s): Karl Thompson, Howard University, Washington DC; Joseph Aubee, Howard University, Washington DC; Jalisa Nurse, Howard University, Washington DC
Fidelity during translation is a crucial factor for the management of gene expression Fidelity is mostly important during the elongation segment of translation. The lack of fidelity during this process can lead to decreased protein expression or abnormal protein folding. Environmental and genetic events can lead to translational infidelity. The presence of RNA modifications has been shown to prevent the translational infidelity events. RNA modifications are defined as chemical additions to one of the four RNA nucleotides, which are adenine, cytosine, guanine, and uridine. The purpose of this experiment was to determine how RNA modifications affect the translational fidelity of the RNA chaperone Hfq in E. coli. It was hypothesized that a numerous amount of RNA modifications was necessary for the translational fidelity of E. coli. To determine the modifications that were necessary for the fidelity of translational elongation, the expression of HFQ was measured with the use of an inducible bacterial reporter gene assay, in the absence of 18 selected RNA modification mutants. As a result of the assay, it was determined that TusA is necessary for Hfq translation and the lack of that modification decreases the translation of Hfq. This experiment is important because further experimentation of RNA modifications in eukaryotic cells can help with the treatment of a rare mitochondrial diseases called MELAS (Mitochondrial Encephalopathy Lactic Acidosis and Stroke like episodes) syndrome.
Funder Acknowledgement(s): Howard University
Faculty Advisor: Karl Thompson, firstname.lastname@example.org
Role: I did the beta-galactosidase assay, grew the strains that contained different mutations of E. coli, and the data analysis.