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RES Modulates Hsp70 in Prostate Cancer Cells

Undergraduate #22
Discipline: Biological Sciences
Subcategory: Cancer Research

Uriel Rose - Alabama State University
Co-Author(s): James Stokes, Sanjay Kumar, and Manoj Mishra, Alabama State University, Montgomery, AL



Hsp70 is highly conserved group of protein expressed in almost all mammalian cells. Normally, it acts as molecular chaperone by assisting proper folding-refolding of a newly formed peptide chain. Resveratrol (3, 4′, 5 trihydroxystilbene) is a naturally occurring phytoalexin produced by some spermatophytes, such as grapevines, in response to injury. Resveratrol (RES) is an antioxidant, anti–inflammatory, anti–proliferative, proapoptotic, and anti–angiogenic compound. The objective of this study was to elucidate the role of Resveratrol and its effect(s) in modulating the expression of hsp70 in murine prostate cancer cells. To test this objective, prostate cancer (PCa) cells were cultured in DMEM/RPMI supplemented with 10% fetal bovine serum (FBS), penicillin 100 IU/ml, streptomycin 100 μg/ml, 0.005mg/ml bovine insulin, and 10nM Dehydroisoandrosterone in 5% CO2 at 370C. PCa cells were harvested by trypsinization after ~80% confluency (at 4-5 passes), counted (105/ml), and incubated with RES. Cell viability and western blot analysis for the expression hsp70 were carried out. Results show that treatment with RES modulates cell viability, expression of Hsp70 in PCa cells. We also found modulation in the expression Hsp70 at genetic level. Thus, RES may be a potential therapeutic regimen to treat this cancer; however, further research is needed.

Funder Acknowledgement(s): This work was supported by: The National Institute of Health grant P20CA192976 (MKM); The National Science Foundation grant 1154214 (MKM).

Faculty Advisor: Manoj Mishra, mmishra@alasu.edu

Role: I cultured prostate cancer (PCa) cells in DMEM/RPMI supplemented with 10% fetal bovine serum (FBS), penicillin 100 IU/ml, streptomycin 100 ?g/ml, 0.005mg/ml bovine insulin, and 10nM Dehydroisoandrosterone in 5% CO2 at 370C. I harvested PCa cells by trypsinization after ~80% confluency (at 4-5 passes), counted (105/ml), and incubated with RES. I did Cell viability and western blot analysis for the expression hsp70.

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This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

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