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Maintaining Habitable Nematode Growth Medium Environments With Anti-fungal Solutions

Undergraduate #53
Discipline: Biological Sciences
Subcategory: Cell and Molecular Biology

Andrew P. Lexington - Harris-Stowe State University
Co-Author(s): Jana Marcette, Harris-Stowe State University, St. Louis, MO



When maintaining C. elegans in a laboratory environment, sterilization can become strenuous. Due to some inevitable errors along with unavoidable environmental conditions, NGM nematode growth medium plates can become populated with unwanted fungus and bacteria from simply being exposed to the air for extended periods of time. This is particularly an issue within undergraduate laboratory research studies in a general classroom setting. This is because many individual experimenters in these settings are novices within sterile laboratory procedure. As a solution, the possibility of discovering a solution that deters the growth of fungus and bacteria without being fatal or disruptive to the C. elegans is the mission of this experiment. We, as students enrolled in Biology Survey Laboratory of Harris-Stowe State University will be following up these results with an exploration of the role of CAMSAP binding partners in this regeneration process. Student-centered pedagogy and engagement in research activities is associated with improved academics, science process skills and retention. A core component of new discovery based introductory laboratory classes is student engagement in faculty-based research. Students work in a collaborative-style biotechnology core research facility concurrently used by faculty and research students. The redesigned program provides the opportunity for students whose interest is peaked in required coursework to continue working closely with faculty as research students.

Funder Acknowledgement(s): This work was supported by a National Science Foundation Targeted Infusion Grant (NSF ID: 1533545) awarded to Dr. Jana Marcette, Harris-Stowe State University.

Faculty Advisor: Jana Marcette, janamarcette@gmail.com

Role: The experiment design and experiment data collection process

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This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

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