Discipline: Chemistry and Chemical Sciences
Subcategory: Biochemistry (not Cell and Molecular Biology and Genetics)
Susan Campbell - Louisiana Tech University
Co-Author(s): Mercede Furr and Thallapuranam Krishnaswamy Suresh Kumar, University of Arkansas, Fayetteville, AR
The purification of proteins for use in research and medical fields is a costly and time consuming procedure when using the current methods available. This research formulates a new purification method for GST-tagged proteins. The fusion protein of GST-cpSRP43 was first overexpressed in E. coli. This protein was then successfully purified following cleavage through the use of heat treatment in order to precipitate the GST-affinity tag. The results of this research are anticipated to make the purification of GST-tagged proteins that are heat stable a much easier and more cost friendly procedure.
Funder Acknowledgement(s): NSF grant CHE-1263119/REU, NIH grant (P30 GM 103450), NSF grant IOS-0842937, DOE grant DE-02-01ER15161.
Faculty Advisor: Thallapuranam Krishnaswamy Suresh Kumar, firstname.lastname@example.org
Role: I lysed the cells and separated the components using centrifugation. I verified the purification of the the affinity protein using a GSH-Sepharose column. I then performed multiple heat treatments and cleavage experiments on the proteins and ran SDS-PAGE gels in order to monitor the results of the results of the experiments.