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Developing an Anther Culture System in Soybeans

Undergraduate #129
Discipline: Biological Sciences
Subcategory: Plant Research

Kiah Ferrell - University of Arkansas at Pine Bluff
Co-Author(s): Thomas Sustich, Martina Garda, Kumar Bade, Yixuan Li, Nathan Williams, Bryant Fong, and Gregory Williams, Arkansas State University, Jonesboro, AR Destiny Jones, University of Arkansas at Pine Bluff



This research was based on an ongoing project with soybeans. Soybean is a crop that provides essential nutrients and health benefits for animals and humans. Soybean seeds are known to contain high amounts of protein used for food and feed. Soybean crops are in big demand globally and farmers need a better cost-effective method to reduce growth time in the field before harvest. We propose to test our hypothesis that more haploid soybeans can be grown from a soybean anther culture system to make a double haploid in one generation to help reduce growth time in the field. Soybean seeds were germinated in growth chambers, monitored and allow to grow for 3-4 weeks. Once, the floral buds were between three to five centimeters, they were pre-shocked in a cold chamber before collection. Overall results showed development of water-like droplet structures in a condensation free environment, which may be indicative of production of early embryonic calli. This may symbolize the presence of the anther wall in the soybean. In conclusion, we believe the water-like droplets may appear before a callus response forms on the cultures.

Funder Acknowledgement(s): Division of Agriculture: Research & Extension: University of Arkansas System, Arkansas Center for Plant Powered Production, Doctoral Molecular Biosciences, United States Department of Agriculture, Arkansas State University: Bridging The Divide, Arkansas State University: College of Agriculture & Technology

Faculty Advisor: Anissa Buckner,

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This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

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