Discipline: Chemistry and Chemical Sciences
Subcategory: Cell and Molecular Biology
Room: Exhibit Hall
Emily Garl - Delaware State University
Cannabinoids have been shown to effectively reduce tumor growth in different in vitro and in vivo cancer models, however, these anticancer effects appear to be dependent on cancer type and drug dose. Understanding how cannabinoids modulate essential cellular processes involved in tumorigenesis, such as the progression through the cell cycle, cell proliferation and cell death, as well as the interactions between cannabinoids and immune system are crucial for improving existing medications and developing new therapeutic approaches. In this study, we will do a comparative analysis of CBD concentration on tumor cell viability. Briefly, tumor cell lines were cultured in medium and supplemented with supplemented with fetal bovine serum, penicillin and streptomycin. All cells were incubated at 37˚C in an atmosphere of 5% CO2. For cell viability staining and DNA isolation, confluent cells were trypsinized and plated onto 6-well-plates (coverslips) and incubated in their respective culture medium Cells of each cell line were cultured and counted at 24 h intervals for 7-day period. Preliminary results confirm that cannabidiol (CBD) reduced tumor growth.
Funder Acknowledgement(s): National Science Foundation (NSF)
Faculty Advisor: Kimberly A. Milligan, email@example.com
Role: The research is ongoing.