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Cannabidiol Concentration on Tumor Cell Viability

Undergraduate #170
Discipline: Chemistry and Chemical Sciences
Subcategory: Cell and Molecular Biology
Session: 1
Room: Exhibit Hall

Emily Garl - Delaware State University


Cannabinoids have been shown to effectively reduce tumor growth in different in vitro and in vivo cancer models, however, these anticancer effects appear to be dependent on cancer type and drug dose. Understanding how cannabinoids modulate essential cellular processes involved in tumorigenesis, such as the progression through the cell cycle, cell proliferation and cell death, as well as the interactions between cannabinoids and immune system are crucial for improving existing medications and developing new therapeutic approaches. In this study, we will do a comparative analysis of CBD concentration on tumor cell viability. Briefly, tumor cell lines were cultured in medium and supplemented with supplemented with fetal bovine serum, penicillin and streptomycin. All cells were incubated at 37˚C in an atmosphere of 5% CO2. For cell viability staining and DNA isolation, confluent cells were trypsinized and plated onto 6-well-plates (coverslips) and incubated in their respective culture medium Cells of each cell line were cultured and counted at 24 h intervals for 7-day period. Preliminary results confirm that cannabidiol (CBD) reduced tumor growth.

Funder Acknowledgement(s): National Science Foundation (NSF)

Faculty Advisor: Kimberly A. Milligan, kmilligan@desu.edu

Role: The research is ongoing.

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This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

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