Discipline: Chemistry and Chemical Sciences
Subcategory: Cancer Research
Room: Exhibit Hall A
Aungelique McGhee - Xavier University of Louisiana
Co-Author(s): Gabrielle Massey, Xavier University Of Lousiana, New Orleans Brandon Dang, Xavier University Of Lousiana, New Orleans Patricia Pham, Xavier University Of Lousiana, New Orleans Xinping Yue, Xavier University Of Lousiana, New Orleans Zhe Wang, Xavier University Of Lousiana, New Orleans VIncent V Nguyen, Xavier University Of Lousiana, New Orleans
A portable solution for early detection of cancers essentially reduces the possibility of death due to cancer progression. Before a prototype of this device can be made, there is a need to address the multiple biomarkers in sufficient amounts and to validate the specificity and sensitivity of disease detection. The biomarker tested in this experiment is the well-studied biomarker SULF1 which is found in every person, but at various concentrations within people with different cancerous diseases. However, there is no efficient way of detecting SULF1 levels in human body fluids currently. Developing a method to monitor efficiently and effectively SULF1 levels would greatly aid in the pursuit of finding a successful way to detect diseases, such as cancer in the breasts or lungs, in its early stages. In this experiment electrochemical method using heparan sulfate proteoglycans (HSPGs) as an anchor was employed to detect the concentration of SULF. Because SULF can transform the 6-O sulfation of HSPGs to OH group, the structure and electrical property of HSPGs could take some changes. Electrochemical impedance spectroscopy was used to monitor the changes of electrical properties of HSPGs with SULF variations. EIS revealed information such as charge, capacity, and degree of phase, we were then able to establish the calibration curve of SULF concentrations, which were used to accuratley detect the SULF activity within human blood samples. In order to study SULF function in carcinoma samples have been tested with this new developed method and will be compared with ELISA. This work offers a new method for SULF activity detection and has great potential in the early detection of cancers. Based on the results, this method of detection has high sensitivity to the reaction of SULF1 enzyme on heparan sulfate. This cancer detecting method proves to be rapid and low-cost with high accuracy. This method is a robust and selective for next POCT cancer diagnosis.
Funder Acknowledgement(s): Support for this work was provided by the Xavier University of Louisiana Summer Undergraduate Research Fellowship program. This work was made possible by funding from the NIMHD-RCMI grant number 5G12MD007595 from the National Institute of Minority Health, Health Disparities and the NIGMS-BUILD grant number 8UL1GM118967 and NSF MCB-1830866. This work also made possible by the Louisiana Cancer Research Consortium (LCRC).
Faculty Advisor: Zhe Wang, email@example.com
Role: I did setup for the experiment, performed the experiment, and treated the data.