• Skip to main content
  • Skip to after header navigation
  • Skip to site footer
ERN: Emerging Researchers National Conference in STEM

ERN: Emerging Researchers National Conference in STEM

  • About
    • About AAAS
    • About the NSF
    • About the Conference
    • Partners/Supporters
    • Project Team
  • Conference
  • Abstracts
    • Undergraduate Abstract Locator
    • Graduate Abstract Locator
    • Abstract Submission Process
    • Presentation Schedules
    • Abstract Submission Guidelines
    • Presentation Guidelines
  • Travel Awards
  • Resources
    • Award Winners
    • Code of Conduct-AAAS Meetings
    • Code of Conduct-ERN Conference
    • Conference Agenda
    • Conference Materials
    • Conference Program Books
    • ERN Photo Galleries
    • Events | Opportunities
    • Exhibitor Info
    • HBCU-UP/CREST PI/PD Meeting
    • In the News
    • NSF Harassment Policy
    • Plenary Session Videos
    • Professional Development
    • Science Careers Handbook
    • Additional Resources
    • Archives
  • Engage
    • Webinars
    • ERN 10-Year Anniversary Videos
    • Plenary Session Videos
  • Contact Us
  • Login

The Effect of Alcohol on the Biogenesis and Composition of Microglia-derived Exosomes

Graduate #21
Discipline: Biological Sciences
Subcategory: Microbiology/Immunology/Virology

Brennetta Crenshaw - Alabama State University
Co-Author(s): Sparkle D.Williams2, B. Sims3, Q.L. Matthews1 1Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, 2Departments of Pediatrics, Neurobiology and Cell, Developmental and Integrative Biology, Division of Neonatology, University of Alabama at Birmingham



Exosomes are small extracellular vesicles that are formed during the maturation of endosomes. The biogenesis of exosome changes based on external factors, such as alcohol exposure. As a small molecule, alcohol can easily cross membrane barriers and reach different parts of the body very quickly. Alcohol interacts with brain receptors, interfering with communication between nerve cells, and suppressing the excitatory nerve pathway. We hypothesize that exosomes protect against alcohol mediated apotosis in microglia cells. Therefore, the purpose of this project was to investigate the effects of alcohol exposure on the biogenesis and composition of exosomes derived from microglia brain cells, BV2. The BV2 cell lines was cultured in exosome-free medium and was either not treated (control) or treated with 50mM or 100mM of alcohol for 24, 48, or 72 hours. The cell morphology was examined through light microscopy. The groups of exosomes were isolated using a series of high-speed ultracentrifugation and quantitated using the Lowry dilution method. NanoSight analysis was used to identify and quantify the size of exosomes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to separate the exosomal proteins based on size and Coomassie blue staining was performed to identify the presence of exosomal proteins each treatment group. Enzyme-linked immunosorbent assay was performed on the exosomes to confirm the presence of exosomal proteins on BV2 cells.
Cell viability decreased after alcohol administration. Cells showed apoptotic behavior after 48-hour and 72-hour treatments after dosing with both concentrations of alcohol. Cells appeared to be shrunken compared to the control untreated cells. The average of three experiments demonstrated through fold change that alcohol treatment at 48 hours at the high dose caused a significant increase of tetraspanin CD63 expression in exosomes. In addition, alcohol treatment after 48 hours caused significant increases of heat shock protein 90 beta compared to the control. At 72 hour treatment with alcohol, we observed a significant increase of cytoskeletal protein actin expression in exosomes when compared to control. Our studies revealed that exosome biogenesis and composition was affected by alcohol treatment. However, more experiments will be conducted to gain additional knowledge on the impact of alcohol on exosome biogenesis in brain resident cells.

Not Submitted

Funder Acknowledgement(s): This work was supported by the American Association of Immunologists Careers in Immunology Fellowship Program (to QLM), Gorgas Memorial Foundation Research Grant Award (QLM). National Science Foundation (NSF) grant HRD 1401075 (to GTJ). National Institutes of Health grant #5R01AI089337-04 (to QLM), and University of Alabama at Birmingham (UAB) Center for AIDS Research (CFAR), an NIH-funded program (P30 AI027767).

Faculty Advisor: Qiana Matthews, qmatthews@alasu.edu

Role: I did majority of the work (cell culture, exosome purification and quantitation, ELISA, etc) on this project.

Sidebar

Abstract Locators

  • Undergraduate Abstract Locator
  • Graduate Abstract Locator

This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

AAAS

1200 New York Ave, NW
Washington,DC 20005
202-326-6400
Contact Us
About Us

  • LinkedIn
  • Facebook
  • Instagram
  • Twitter
  • YouTube

The World’s Largest General Scientific Society

Useful Links

  • Membership
  • Careers at AAAS
  • Privacy Policy
  • Terms of Use

Focus Areas

  • Science Education
  • Science Diplomacy
  • Public Engagement
  • Careers in STEM

Focus Areas

  • Shaping Science Policy
  • Advocacy for Evidence
  • R&D Budget Analysis
  • Human Rights, Ethics & Law

© 2023 American Association for the Advancement of Science