Discipline: Biological Sciences
Subcategory: Microbiology/Immunology/Virology
Christian Lee - Alabama State University
Co-Author(s): Sameer Joshi,Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL; Vida A. Dennis, Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL; Komal Vig Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL; Karanam Balasubramanyum, Department of Biology and Cancer Research, Tuskegee University, Tuskegee, AL; Shree R. Singh, Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL
Purpose: E.coli can infect many parts of the human body such as intestinal tract, urinary tract and skin (Hilbert 2013). There are a number of treatments that are currently available for the skin infections and mostly fall in the antibiotic class of drugs; however nanoparticles such as gold and silver are also under evaluation for their efficacy against E.coli (Franková, Pivodová et al. 2016; Balasubramanyam, Altaf et al. 2004). In this study gold nanoparticles (GNPs) and peptide garcinole (GC) are being tested for their efficacy to stop the E.coli spread when in contact with human keratinocytes. Method: Confluent human keratinocytes of when exposed to GNPs and GC to evaluate their toxicity. The MTT assay with varying concentrations of GNPs and GC. Also, the minimum inhibitory concentration (MIC) assay was also performed to determine the optimum concentration for GC. Results: The MIC assay preformed with GC shows prominent antimicrobial effects against E.coli where GC concentration of 0.1 mM shown 40 % bacterial inhibition. Whereas, the MTT assay shows that 0.08 mM GC and 1 nM GNPs are better with over 60 % cell viability. Conclusion: The initial aim of the study was to observe the antimicrobial effects of GC against E.coli and it was observed that GC can inhibit E-coli. GC found marginally toxic but the cell viability for concentrations below 0.08 mM is over 60 %. GNPs on the other hand found dependable with over 90 % cell viability. Further in this research conjugation of GC and GNPs will be done. It is considered that the conjugation will reduce the toxicity of GC and the conjugation product could show antimicrobial effect. References: Balasubramanyam, K., M. Altaf, et al. (2004). “Polyisoprenylated benzophenone, garcinol, a natural histone acetyltransferase inhibitor, represses chromatin transcription and alters global gene expression.” Journal of Biological Chemistry 279(32): 33716-33726. Franková, J., V. Pivodová, et al. (2016). “Effects of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model.” Journal of applied biomaterials & functional materials 14(2). Hilbert, D. W. (2013). Uropathogenic Escherichia coli: the pre-eminent urinary tract infection pathogen, Nova Publishers.
Abstrat-Christian Lee-ERN-2017.docxFunder Acknowledgement(s): The project is funded by NIH under the Research Initiative for Scientific Enhancement (RISE) program (Grant IR2SGM10699 5-01).
Faculty Advisor: Dr. Komal Vig, komalvig@alasu.edu
Role: Method: Confluent human keratinocytes of when exposed to GNPs and GC to evaluate their toxicity. The MTT assay with varying concentrations of GNPs and GC. Also, the minimum inhibitory concentration (MIC) assay was also performed to determine the optimum concentration for GC.