Discipline: Biological Sciences
Subcategory: Cancer Research
LaTerrian Wiley - Jarvis Christian College
Co-Author(s): Shakhawat Bhuiyan, Jarvis Christian College, Hawkins, TX
D-Allose, a rare aldo-hexose distributed rarely in nature substantially inhibited segmented neutrophil production and lowered platelet counts without other detrimental clinical effects (US pat. no. 5620960, 1997). Recently, D-Allose has been investigated to inhibit various cancer cells proliferations, indicating D-allose induces anti-cancer effects and also shown selective cytotoxicity against human cancer cell lines. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old Caucasian woman (American Type Culture Collection). MCF-7 cells are useful for in vitro breast cancer studies as a result of the cell line retaining several ideal characteristics particular to the mammary epithelium. When MCF-7 cells are grown in vitro, the cell line is capable of forming domes and the epithelial like cells in monolayers. MCF-7 cells are widely used in many different studies and have been treated with many different anticancer agents such as nanoparticles and nonconventional treatment. In this study, we hypothesized whether D-allose had anti-cancer effects on MCF-7 breast cancer cells. The MCF-7 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1.0% penicillin/streptomycin (Invitrogen) at 370 C in 5% CO2/95% air. To investigate the anti-cancer effects of D-allose, we treated MCF-7 cells with different concentrations of D-allose ranging from 2mM to 100mM for various time periods (24h, 48h and 72h), and viability of cells were assayed by MTS method. The results showed the inhibition of cell growth in cancer cells treated with D-allose for 24h as compared to their respective controls, and the strongest inhibition of 65% was found in cells at concentration of 100 mM D-allose. The proteins from treated cells were extracted and measured. The protein concentrations were reduced significantly in the higher concentrations of D-Allose. Furthermore, Western blot analysis of proteins will be performed to analyze the signaling proteins associated with the cytotoxic effects of D-allose.Not Submitted
Funder Acknowledgement(s): Acknowledgement of Funders: This research was funded by a NSF/HBCU-UP grant awarded to Dr. Shakhawat Bhuiyan PhD, Professor of Biology, Jarvis Christian College, Hawkins ,Texas.
Faculty Advisor: Shakhawat Bhuiyan Ph.D, Sbhuiyan@jarvis.edu
Role: Grow the breast cancer cells and maintaining the cells and counting cells by hemocytometer. Investigate the anti-cancer effects of D-allose,we treated MCF-7 cells with different concentrations of D-allose ran and viability of cells were assayed by MTS method. The results showed the inhibition of cell growth in cancer cells treated with D-allose as compared to their respective controls, and the strongest The proteins from treated cells were extracted and measured.