Quantitative Proteomic Analysis of Mice Kidney Reveals an Effect of High Fat Diet on Protein Expression

Undergraduate #44
Discipline: Biological Sciences
Subcategory: Cell and Molecular Biology

Damiesha Bryant - Philander Smith College

As obesity remains prevalent in the western world, it is critical to understand how excess visceral adiposity alters the function of other organs. Obesity increases the risk of developing degenerative conditions such as hypertension and Chronic Kidney Disease. Dysfunctions in the regulation of the renin-angiotensin-aldosterone system has been shown to promote renal disorders. High fat diet induced obesity has also been shown to lead to hypertension due to both excessive visceral adipose tissue surrounding the kidneys and the elevation of renal tubular sodium reabsorption. Not only does a high fat diet promote hypertension, but also it encourages an increased fatty acid oxidation and decreased fatty acid synthesis. In this study, using mass spectrometry based proteomic analysis, we evaluated the effects of high fat diet (HFD) induced obesity on the mouse kidney protein expression to gain insight into how obesity may lead to Chronic Kidney Disease. Our findings suggest that long-term consumption of a high fat diet may cause decreased expression of proteins in the kidney such as Acetyl Carboxylase I and II, Renin, and Fatty Acid Synthase while proteins such as CYP450 A410, CYP450 A414, and Angiotensinogen may increase in expression.

Not Submitted

Funder Acknowledgement(s): Special thanks to Dr. Lawrence J. Mandarino for use of lab and assistance with this study. Funding provided by Louis Stokes Alliances for Minority Participation (LSAMP).

Faculty Advisor: Dr. Jocelyn Moore, jamoore@philander.edu

Role: Quantitative Proteomic Data Analysis