• Skip to main content
  • Skip to after header navigation
  • Skip to site footer
ERN: Emerging Researchers National Conference in STEM

ERN: Emerging Researchers National Conference in STEM

  • About
    • About AAAS
    • About NSF
    • About the Conference
    • Project Team
    • Advisory Board
  • Conference
  • Abstracts
    • Abstract Submission Process
    • Abstract Submission Guidelines
    • Presentation Guidelines
  • Travel Awards
  • Resources
    • Award Winners
    • Code of Conduct-AAAS Meetings
    • Code of Conduct-ERN Conference
    • Conference Agenda
    • Conference Materials
    • Conference Program Books
    • ERN Photo Galleries
    • Events | Opportunities
    • Exhibitor Info
    • HBCU-UP PI/PD Meeting
    • In the News
    • NSF Harassment Policy
    • Plenary Session Videos
    • Professional Development
    • Science Careers Handbook
    • Additional Resources
    • Archives
  • Engage
    • Webinars
    • 2023 ERN Recap Video
    • ERN 10-Year Anniversary Videos
    • Plenary Session Videos
  • Contact Us
  • Login

Chemoenzymatic Synthesis of a Heparan Sulfate Library

Undergraduate #2
Discipline: Biological Sciences
Subcategory: Biochemistry (not Cell and Molecular Biology and Genetics)

Michelle Bessiake - Texas Southern University
Co-Author(s): Matt Suflita, Fuming Zhang, and Robert Lindhardt, Rensselaer Polytechnic Institute, Albany, NY



In this study, we focus on synthesizing a heparan sulfate (HS) library by using biosynthetic enzymes to produce different sulfation patterns. Heparan sulfate is a complex polysaccharide composed of repeating dissaccharde units (L-iduronic aid and glucosamine) with variable sulfation patterns. Using the E. coli K5 capsular polysaccharide heparosan as the starting material, the heparosan is chemically N-sulfated. Once the resulting N- sulfo heparosan is formed it is treated with various combinations of HS biosynthetic enzymes including: 2-Osulfotranferase-1 (2-OST-1), C5 epimerase, 6-OST-1, 6-OST-3, and 3-OST-1. The resulting HS library was then analyzed by reverse phase ion pairing chromatography – mass spectrometry (RPIP-MS) to determine the sulfation type and composition. For future endeavors, this heparan sulfate library will be used to study the structure-activity relationship between HS sulfation patterns and fibroblast growth factor signaling efficiency

Funder Acknowledgement(s): Dr. Robert Lindhardt

Faculty Advisor: Bobby Wilson,

Sidebar

Abstract Locators

  • Undergraduate Abstract Locator
  • Graduate Abstract Locator

This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

AAAS

1200 New York Ave, NW
Washington,DC 20005
202-326-6400
Contact Us
About Us

  • LinkedIn
  • Facebook
  • Instagram
  • Twitter
  • YouTube

The World’s Largest General Scientific Society

Useful Links

  • Membership
  • Careers at AAAS
  • Privacy Policy
  • Terms of Use

Focus Areas

  • Science Education
  • Science Diplomacy
  • Public Engagement
  • Careers in STEM

Focus Areas

  • Shaping Science Policy
  • Advocacy for Evidence
  • R&D Budget Analysis
  • Human Rights, Ethics & Law

© 2023 American Association for the Advancement of Science