Discipline: Biological Sciences
Subcategory: Biomedical Engineering
Savon Vigil - Eastern Connecticut State University
Co-Author(s): Maumita Bhattacharjee
The chorion is the outermost thin membrane that surrounds the fetus and contains villi that connect to mothers blood vessels in order to form the fetal part of the placenta. This membrane consists of two layers- the trophoblastic ectoderm and extraembryonic mesoderm which allows the development of these villi, which becomes vascularized by allantoic vessels. The object of this experiment was to determine whether or not an injectable chorion based hydrogels could be developed for cell delivery application that could support MC3T3 survival. Depending on the application, Chorion gels can be used for slow delivery (high concentration) or fast delivery (low concentration). These hydrogels can be used with a variety type of stem cell such as adipose derived stem cells or embryonic stem cells. With success, this method could be used any application for bioengineering and tissue regeneration.
Funder Acknowledgement(s): Uconn Health
Faculty Advisor: Maumita Bhattacharjee, email@example.com
Role: I followed the lab procedures outlined for me by Maumita and recorded all data that was collected. With the assistance of my advisor, I helped with the isolation of chorion, decontamination, decellularization, and lyophilization of the tissue. Once this was done, i assisted with the gel formation and performed live/dead assay to quantify cell viability at different time intervals.