• Skip to main content
  • Skip to after header navigation
  • Skip to site footer
ERN: Emerging Researchers National Conference in STEM

ERN: Emerging Researchers National Conference in STEM

  • About
    • About AAAS
    • About the NSF
    • About the Conference
    • Partners/Supporters
    • Project Team
  • Conference
  • Abstracts
    • Undergraduate Abstract Locator
    • Graduate Abstract Locator
    • Abstract Submission Process
    • Presentation Schedules
    • Abstract Submission Guidelines
    • Presentation Guidelines
  • Travel Awards
  • Resources
    • Award Winners
    • Code of Conduct-AAAS Meetings
    • Code of Conduct-ERN Conference
    • Conference Agenda
    • Conference Materials
    • Conference Program Books
    • ERN Photo Galleries
    • Events | Opportunities
    • Exhibitor Info
    • HBCU-UP/CREST PI/PD Meeting
    • In the News
    • NSF Harassment Policy
    • Plenary Session Videos
    • Professional Development
    • Science Careers Handbook
    • Additional Resources
    • Archives
  • Engage
    • Webinars
    • ERN 10-Year Anniversary Videos
    • Plenary Session Videos
  • Contact Us
  • Login

Analysis of Isoflavones in Soybeans using LC-MS and UV Spectral Analysis

Undergraduate #137
Discipline: Biological Sciences
Subcategory: Plant Research

Rita Dill - Bowie State University
Co-Author(s): Anne Osano1. and Devanand Luthria2. 1- Department of Natural Sciences ; Bowie State University, MD 20715; 2- Food Composition and Methods Development Laboratory Chemistry, Beltsville, MD



Isoflavones are a class of bioactive phytoestrogens which are plant-derived compounds with estrogenic activity. Soybeans, (Glycine max), provides the richest source of natural isoflavones in the human diet. The purpose of this research was to analyze the changes in the isoflavone content in soybean. It is hypothesized that mutations will impact the isoflavone profiles and content in soybeans. Isoflavones were extracted from ground soybeans using EtOH:H2O: DMSO [79:20:1] with ultrasonic assisted extraction. The analysis was performed using Liquid Chromatography- Mass Spectroscopy (LC-MS). The major isoflavones were identified as daidzein malonyl glucoside and genistein malonyl glucoside. In addition, trace quantities of genistein, daidzein, daidzin, glycitin and biochanin were identified as well. The analysis showed that there were insignificant differences in the isoflavones’ profiles; however, the significant changes in the quantity of isoflavones were observed in different mutated soybean samples. It is planned to continue our research on the identification of four additional components. In addition, the long term goals of this research will be to determine these changes over multiple growing seasons in different environments.

Rita abstractdave mod.doc

Funder Acknowledgement(s): USDA, Food Composition and Methods Development Laboratory Chemistry, Beltsville, MD.

Faculty Advisor: Dr. Anne Osano, asano@bowiestate.edu

Role: I did not radiate the soy beans. The soy beans were provided by a separate lab. I had assistance in identifying the isoflavones. With the exception of those two components I completed all other portions of the research.

Sidebar

Abstract Locators

  • Undergraduate Abstract Locator
  • Graduate Abstract Locator

This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

AAAS

1200 New York Ave, NW
Washington,DC 20005
202-326-6400
Contact Us
About Us

  • LinkedIn
  • Facebook
  • Instagram
  • Twitter
  • YouTube

The World’s Largest General Scientific Society

Useful Links

  • Membership
  • Careers at AAAS
  • Privacy Policy
  • Terms of Use

Focus Areas

  • Science Education
  • Science Diplomacy
  • Public Engagement
  • Careers in STEM

Focus Areas

  • Shaping Science Policy
  • Advocacy for Evidence
  • R&D Budget Analysis
  • Human Rights, Ethics & Law

© 2023 American Association for the Advancement of Science