Discipline: Chemistry and Chemical Sciences
Subcategory: Plant Research
Room: Exhibit Hall A
Tamika Manns - St. Philip's College
Co-Author(s): Julie Hill
Bacteria resistance to common antibiotics has led to the search for new sources to fight health issues. 80% methanolic extraction of H. rosa sinensis leaves (regionally grown) were prepared following the methodology described by Abdallah (2016), and standardized by Reyes & Hill in 2019, for St. Philip’s College project Studying of Bioactive Compounds from Regional Species. Extracts were filtered and concentrated in vacuo at 40 °C and low pressure. Blood agar inoculation and incubation (37 °C) of five bacteria strains were tested with the extract by disc diffusion. The results of this study demonstrated antibacterial activity on all bacteria strains, with a zone of inhibition of 22.8±3.6 for Proteus vulgaris; 22.5±3.6 for Staphylococcus aureus; 23.5±3.7 for Escherichia coli; 21.5±6.1 for Serratia marcescens and 20.5±6.9 for Bacillus subtilis (n=15 for all experiments). Future research will include the synergistic effects on bacteria from flowers and leaves of H. rosa sinensis. The project seeks to use the research experience as an educational tool for students of St. Philip’s College.
Funder Acknowledgement(s): St. Philip's College; CIMA~LSAMP
Faculty Advisor: Dr. Nava-Fischer, Carmen, email@example.com
Role: I assisted in many areas in this project, from cleaning, sorting, drying and grinding the leaves to running the filtration, running the rotovapor and even streaking the blood agar plates with the bacterial strains. I also measured and photographed some of the zone of inhibitions on the samples.