Discipline: Biological Sciences
Subcategory: Pollution/Toxic Substances/Waste
Noura Abdulrahman - Texas Southern University
Co-Author(s): Ayat Ali, PhD., Texas Southern University, Houston, TXShishir Shishodia, PhD., Texas Southern University, Houston, TXNabras Abdulrahman, MS., Texas Southern University, Houston, TX
The Study of Indoor Dust Trace Metal and Organic Contaminants Toxicity Impact on Normal Human Epithelial Colon Cell CCD841 Indoor dust stands out as a burgeoning challenge in society due to its implication on people’s health. Exposure to toxic dust particles occurs in different ways that include inhalation, ingestion, and dermal contact. In the hindsight, many people spend most of their time indoors exposing themselves to high levels of dust. Notably, different groups of individuals present varied risk levels to the toxicity of particles with vulnerable persons being susceptible to adverse effects of these contaminants. This includes the elderly, children, and immunocompromised individuals. The study examines the impacts of two variables that are trace elements (TMD) and organic contaminants (OD) on a normal human colon cell (CCD841) that is exposed to indoor dust. In this case, cultures of the cells were made and treated with varying concentrations of TMD and OD. Then, different factors were observed to highlight the impact of each sample on the cultured cells. Consecutively, viability, apoptosis, cytotoxicity, oxidative stress, and inflammation were observed. The methods used in the experiment included the performance of MTT assay, protease cell viability, apoptosis 3/7 caspase, LDH release, reactive oxygen species, and inflammasome caspase-1 assay. In this case, two variables were treated to these experiments while varying their concentrations.The findings indicated significant figures when both variables had higher concentrations. This supported the research hypothesis where prolonged exposure to indoor dust results in huge damage to the body, specifically the normal colon cells for this study. The research points out to the importance of averting indoor air pollution. The effects of indoor dust samples in activation of oxidative stress by measuring the generation of reactive oxygen species (ROS), was noticeable which suggest weakening the immune system, and the human body becomes an easy target to invade. Inflammasome-Caspase-1 also was induced when being exposed by both dust samples. Although, both variables TMD and OD have a positive correlation, The results shows that a significant change in the culture cells meant an increase in the concentration of both TMD and OD, and it is hard to be determined which one of these two samples is more toxic to normal human colon cells; but if they both are combined and penetrate at a cellular level, there is a high chance to cause a serious harm.HypothesisIf normal human colon (CCD 841) cells are exposed to dust particles, there will be a reduction in cells viability, increase in cytotoxicity, cellular death, reactive oxygen species, and inflammation.Amis1.Examine the effects of dust samples on CCD841 cell viability using MTT and GF-AFC Assays2.Examine the effects of dust samples on CCD841 cell cytotoxicity using LDH release Assay.3.Examine the effects of dust samples on CCD841 cell activation of reactive oxygen species.4.Examine the effects of dust samples on CCD841 cell inflammatory using inflammasome caspase 1 Assay.Method 1.Indoor Dust Samples & Preparation2.Cell culture of CCD8413.Cell Viability Assays (MTT & Protease Viability)4.Cell Cytotoxicity Assay using Lactate Dehydrogenase Release5.Cell Death by Apoptosis Assay (Caspase-Glo 3/7 Activation) Overview6.Extracellular Reactive Oxygen Species Detection Using (ROS-Glo™ H2O2 Assay)7.Inflammatory Assay Using Caspase 1-Inflammasome Activity Overview8.Software and Statistical Analysis to Obtain ExperimentThe Study Summary and Results ConclusionThis study was designed to examine the effect of trace elements and organic contaminants dust in indoor dust on normal human colon cells. These cells were cultured, treated by both types of dust samples using different elevated concentrations, tested their viability, cell death mechanism, cytotoxicity, oxidative stress, and inflammation reaction by preforming MTT assay, protease cell viability, apoptosis 3/7 caspase, LDH release, Reactive oxygen species, and inflammasome caspase-1 assay respectively. After preforming standard deviation to statically analyze the data by using GraphPad Prism software generation 9, and probability value was calculated, the results were significant at higher concentration for the above reactions, and it supported the study hypothesis. Finally, the correlation between the two types of dust were used was about the same in terms of their toxicity affects, and it is left for future studies. Future Research Studies: Impact of Indoor Dust on Normal Human Skin Cells e.g., HEKA and HEMA.
Funder Acknowledgement(s): NSF- RISE program
Faculty Advisor: Shishir Shishodia, firstname.lastname@example.org
Role: 1. Performed the experiment of this research as follows: Indoor Dust Samples & PreparationCell culture of CCD841Cell Viability Assays (MTT & Protease Viability)Cell Cytotoxicity Assay using Lactate Dehydrogenase ReleaseCell Death by Apoptosis Assay (Caspase-Glo 3/7 Activation) OverviewExtracellular Reactive Oxygen Species Detection Using (ROS-Glo™ H2O2 Assay)Inflammatory Assay Using Caspase 1-Inflammasome Activity Overview2. Analyzed the collected data from the experiment by using software and statistical Analysis 3. Translating the promising results.