Room: Exhibit Hall A
Arris Thornton - Alabama State University
Co-Author(s): Shreekumar Pillai, Alabama State University, Montgomery, AL; Shree Singh, Alabama State University, Montgomery, AL; Sapna Jain, Alabama State University, Montgomery, AL
Antimicrobial peptides(AMPs) have a great potential as an alternative for the treatment of various bacterial infections and diseases. The aim of the work is to explore the potential of polymer encapsulated Lysobactin and polymixen B nanopeptide hydrochloride against bacteria. Various approaches will be compared to evaluate encapsulation efficiency, AMPs activity and protection against the destructive enzyme proteases. Starch nanoparticles, prepared by ?green chemistry? methods have been used to encapsulate antibiotics. It is hypothesized that hydrolysis of starch produces dextrin and maltose that increases the osmotic effect of the media and hence increased antibacterial activity of the Antibiotic-starch conjugates (ASN). ASN were characterized by FT-IR, SEM, Zeta sizer and LIBS. Minimum inhibitory concentration(MIC) of the ASN conjugates was found out by Kirby Bauer disk diffusion assay. Different concentration of starch loaded with antibiotics were tested against multidrug resistant, Staphlococcus aures. The concentration 1.5?g ASN was found to be effective against the bacteria. Starch nanoparticles can be successfully used for encapsulation of multiple antibiotics, AMPs and drugs to achieve better inhibition without the complication of resistivity. Encapsulation with chitin and chitosan will be tried to evaluate peptide stability and efficiency. In brief polymer capsule-based platform appears suitable for AMPs delivery.
Funder Acknowledgement(s): NSF LSAMP
Faculty Advisor: Dr. Sapna Jain, firstname.lastname@example.org
Role: I was involved in all parts of my research, but specifically I was involved characterization using FT-IR, SEM, Zeta-Sizer, and LIBS techniques. I prepared nano-particles using different concentrations which were loaded all with antibiotics. I also did encapsulation studies.