Discipline: Biological Sciences
Subcategory: Cancer Research
Justin Sharp - Jarvis Christian College, Hawkins, Texas
Co-Author(s): Shakhawat Bhuiyan PhD, Jarvis Christian College, Hawkins, TX
Ultra-violet (UV) radiation induced mutation on biological cells develops a different behavior on normal cells with radiation. Radiation has been used beneficially to inhibit the effects of cancerous cells from proliferating. Some forms of radiation treatment do not force all cells into apoptosis leaving them in an irreparable state to replicate and spread mutation. Treatment was done to determine the time it would take for TK6 human lymphoblastiod cells (American Type Culture Collection) to undergo mutation and morphological changes. The TK6 cells at various concentrations were exposed with varying lengths of UV radiation and then grown for different time intervals. The Tk6 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1.0% penicillin/streptomycin (Invitrogen) at 37 degrees C in 5% CO2/95% air. The viability of the treated and untreated cells was measured by Trypan blue using a Hemocytomer. Cells were treated with UV radiation at varying lengths of times ranging from 1 minute to 5 minutes and then were incubated a further 1 to 5 days to investigate the viability and cellular morphology. Cells at different concentrations ranging from 1 to 5 million cells/ml were treated with the corresponding times previously stated. The most conclusive results showed that cells that underwent a lower radiation length and, had a higher cell count were more likely to show significant morphological changes. Cells that start with a lower cell count at the same radiation length showed that morphology was less likely to happen, and apoptosis would occur more rapidly.
Funder Acknowledgement(s): This research was funded by a NSF/HBCU-UP grant awarded to Dr. Shakhawat Bhuiyan PhD, Professor of Biology, Jarvis Christian College, Hawkins Texas.
Faculty Advisor: Shakhawat Bhuiyan PhD, Sbhuiyan@jarvis.edu
Role: All parts of this research were preformed by myself.