Discipline: Biological Sciences
Subcategory: Cell and Molecular Biology
Room: Exhibit Hall A
Mary C. Butler - Truman State University
Co-Author(s): Co-Authors: Madison T. Green, University of Missouri, Columbia, MO; Rachel E. Martin, University of Missouri, Columbia, MO; Brittney Marshall, University of Missouri, Columbia, MO; Tess Willemse, University of Missouri, Columbia, MO; Jessica A. Kinkade, University of Missouri, Columbia, MO; Jiude Mao, University of Missouri, Columbia, MO; and Cheryl S. Rosenfeld, University of Missouri, Columbia, MO
Bisphenol A (BPA) is a prevalent household chemical found in a variety of different products. Genistein (GEN) is a phytoestrogen/ isoflavone found in soy products, both are considered endocrine disrupting chemicals (EDC). Exposure to either of these chemicals may induce changes in messenger RNAs and microRNAs in the hypothalamic brain region that we hypothesized could affect later behavioral responses. Experiments were thus done to determine whether developmental exposure to BPA or GEN affects later hypothalamic gene expression profiles. To test the effects of BPA and GEN on behavioral and metabolic patterns and hypothalamic gene expression profiles, dams were provided two weeks prior to conception and throughout gestation and lactation one of four different diets: AIN (control), lower dose BPA supplemented diet (5mg/kg feed weight), higher dose BPA supplemented diet (50 mg/kg feed weight), or GEN (250 mg/kg feed weight). A month after birth, the pups are weaned from their parents and placed on the AIN diet. At 180 days of age, various behaviors were analyzed. Body composition of the mice was also measured. qPCR was done on the hypothalamus tissue of the mouse brains. A total of ten genes were tested: Esr1, Esr2, Dnmt3a, Dnmt3b, Avp, Bdnf, Kiss1, Lepr, Oxtr, and Gnrh. There were also four miRNAs being tested: 7a, 9, 153, and 181a. The study found that mice exposed to the GEN and LD BPA mice were less social than AIN control mice. After analyzing the data for the metabolic chamber, it was found that the average respiratory quotient (RQ) for all three of the test diets was higher than that of the control diet. This suggests that the test mice are metabolizing carbohydrates rather than fats. The GEN mice also had greater physical activity than the AIN control mice. The qPCR results showed that Esr2, Avp, Kiss1, Lepr, and miRNA181a were elevated in LD BPA mice. The expression of miRNA153 was significantly decreased for the California mice offspring exposed to the LD BPA diet. Taken together, results show that developmental exposure to GEN and LD BPA affects metabolic, behavioral, and hypothalamic gene expression patterns, which could have important ramifications for humans. Further research will be done to analyze the gene expression in the hippocampus as well as other behavioral data.
Funder Acknowledgement(s): This study was supported, in part, by the Missouri Louis Stokes Alliance for Minority Participation (MoLSAMP), Truman State University, Kirksville, MO; This study was supported, in part, by a grant from NIEHS awarded to Cheryl Rosenfeld PhD, University of Missouri, Columbia, MO.
Faculty Advisor: Dr. Cheryl S. Rosenfeld, email@example.com
Role: The main part of the research that I did was RNA isolation, reverse transcription, and qPCR. I also helped with animal care, running of behavioral tests, coding animal behaviors, tissue collection and isolation, and analysis of results.