Emerging Researchers National (ERN) Conference

nsf-logo[1]

  • About
    • About AAAS
    • About the NSF
    • About the Conference
    • Partners/Supporters
    • Project Team
  • Registration
    • Conference Registration
    • Exhibitor Registration
    • Hotel Reservations
  • Abstracts
    • Abstract Submission Process
    • Presentation Schedules
    • Abstract Submission Guidelines
    • Presentation Guidelines
    • Undergraduate Abstract Locator (2020)
    • Graduate Abstract Locator (2020)
    • Faculty Abstract Locator (2020)
  • Travel Awards
  • Resources
    • App
    • Award Winners
    • Code of Conduct-AAAS Meetings
    • Code of Conduct-ERN Conference
    • Conference Agenda
    • Conference Materials
    • Conference Program Books
    • ERN Photo Galleries
    • Events | Opportunities
    • Exhibitor Info
    • HBCU-UP/CREST PI/PD Meeting
    • In the News
    • NSF Harassment Policy
    • Plenary Session Videos
    • Professional Development
    • Science Careers Handbook
    • Additional Resources
    • Archives
  • Engage
    • Webinars
    • Video Contest
    • Video Contest Winners
    • ERN 10-Year Anniversary Videos
    • Plenary Session Videos
  • Contact Us
  • App View

Construction of a LuxAB Promoter Probe to Assess Light:Dark Responsive Promoters in Synechocystis sp. PCC6803

Undergraduate #93
Discipline: Biological Sciences
Subcategory: Genetics

Katelyn Oliver - Colorado State University
Co-Author(s): Allison Zimont and Christie Peebles, CSU, Fort Collins, CO



Cyanobacteria are photosynthetic bacteria which harness sunlight and atmospheric CO2 to grow. This property makes them a promising candidate for the production of renewable energy in the form of biofuels. It is becoming more important to expand the molecular toolbox for cyanobacteria to aid in the creation of economically viable fuels. Our lab aims to construct plasmids for the use in cyanobacteria to develop enhanced gene expression control, to ultimately increase bio-fuel production. As a proof of concept, we utilized our knowledge of promoter probes to modify the plasmid pAM1580 to enable the study of the light/dark cycles of Synechocystis sp. PCC 6803. We chose the plasmid pAM1580 to modify due to it containing the LuxAB gene and working well in Synechocystis sp. PCC 6803. Molecular cloning and site directed mutagenesis was used to insert three promoters shown to be active during either the light cycle or dark cycle. This presentation will focus on our research efforts to date.

Funder Acknowledgement(s): National Science Foundation grant number #1332404

Faculty Advisor: Christie Peebles,

ERN Conference

Celebrating 10 years of ERN!

What’s New

  • Webinars
  • Events|Opportunities
  • AAAS CEO Comments on Social Unrest, Racism, and Inequality
  • Maintaining Accessibility in Online Teaching During COVID-19
  • In the News
  • #ShutDownSTEM
  • HBCU/CREST PI/PD Meeting

Conference Photos

ERN Conference Photo Galleries

Awards

ERN Conference Award Winners

Checking In

Navigation

  • About the ERN Conference
  • Partners/Supporters
  • Abstracts
  • Travel Awards
  • Conference Registration
  • Exhibitor Registration
  • Hotel Reservations

nsf-logo[1]

This material is based upon work supported by the National Science Foundation (NSF) under Grant No. DUE-1930047. Any opinions, findings, interpretations, conclusions or recommendations expressed in this material are those of its authors and do not represent the views of the AAAS Board of Directors, the Council of AAAS, AAAS’ membership or the National Science Foundation.

AAAS

1200 New York Ave, NW Washington,DC 20005
202-326-6400
Contact Us
About Us

The World's Largest General Scientific Society

Useful Links

  • Membership
  • Careers at AAAS
  • Privacy Policy
  • Terms of Use

Focus Areas

  • Science Education
  • Science Diplomacy
  • Public Engagement
  • Careers in STEM

 

  • Shaping Science Policy
  • Advocacy for Evidence
  • R&D Budget Analysis
  • Human Rights, Ethics & Law
© 2021 American Association for the Advancement of Science