Discipline: Biological Sciences
Subcategory: Cancer Research
Room: Exhibit Hall A
Justin Sharp - Jarvis Christian College
Co-Author(s): Dr. Shakhawat Bhuiyan; Dr. Glendora Carter
Nanoparticles and the use of nanotechnology now play a major role in biomedical research. Recently nanoparticles have been used for targeted drug delivery, cancer immunotherapy and help with disease diagnosis and treatment. Palladium is the 46th element on the periodic table and is a catalyst for hydrogenation. There have been very few studies carried out on Palladium as far as nanotechnology uses. Gardenia Jasminoide Ellis is better known as Jasmine fruit and is said to treat many mental and physical disorders and is even said to inhibit the growth of cancer cells. The objective of this research was to analyze the effects of synthesized palladium nanoparticles from Gardenia Jasminoide Ellis fruits on human cancer Hela cell line. Hela cells originated from Henrietta Lacks, who was diagnosed with cervical cancer was treated but later died from the aggressive form of cancer. The cells originated from John Hopkins hospital where she had gone to get her treatment. Discovered by Dr. Gregory Gey who was researching methods of immortalizing cells long enough to experiment in vitro. In this study, palladium nanoparticles were synthesized using 8.5 ml of crude extract of the jasmine fruit to 4 ml of palladium chloride (0.075M) solution in a reduction method. The UV-vis spectroscopy was used to find the absorbance at a set wavelength (236nm) to determine the concentration of palladium nanoparticles. The calculated concentration of palladium nanoparticles in the stock solution was 37.77nM, which was used to determine how effective it would be on the cancer cells. The cells were treated with varying concentrations of palladium nanoparticles (0.37nM, 0.63nM and 0.88nM) and the data was recorded for the three-day treatment process. The treatment was carried out using a six well plate with 300,000 cells/well and incubated for 48 hours before being treated with the nanoparticles. The results showed that the concentration of palladium nanoparticles at 0.88nM inhibited 55% viability of the cancer cells after 48 hours of treatment. With continued efforts and research development in nanotechnology, palladium nanoparticles could be a vital part in biomedical applications.
Funder Acknowledgement(s): NSF; Welch grant
Faculty Advisor: Shakhawat Bhuiyan, email@example.com
Role: I contributed to all parts aside from the purchasing of material and approval of research.